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Protein scaffold interaction12/12/2022 ![]() The scaffold proteins exert their effects through simple tethering of signaling proteins, properly orienting target proteins, or allosteric assembly of pathway components. Scaffold proteins act as “molecular glue”, linking multiple components in a phosphorylation-dependent signaling pathway together to facilitate signal transduction, and as such play a crucial role in the regulation of signaling cascades. It is believed that the answer may partially lie in the existence of scaffold proteins. The emergence of complex signaling networks prompts the question of specificity, and understanding how individual signals are transduced to arrive at specific outputs is of great importance to the biological community. Many of these signaling pathways share common signaling components and are subject to extensive cross-regulation. Biological systems contain a large number of phosphorylation-related signaling pathways. MEK1 then phosphorylates and activates MAPK ERK1/2. One well-known example is the RAS-ERK pathway, in which a small G-protein RAS activates MAP3K RAF, which then phosphorylates and activates MAP2K MEK1 (MAPKK1). In the context of a signaling pathway, a relay of phosphorylation events could allow the transmission of extracellular signals to intracellular targets. Phosphorylation of a protein may result in a conformational change in its structure, recruitment of binding partners or change of localization, leading to its activation or deactivation. Protein phosphorylation and dephosphorylation is an important means of protein regulation that occur in both prokaryotic and eukaryotic organisms. ![]() The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Ĭompeting interests: The authors have declared that no competing interests exist. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are creditedĭata Availability: All relevant data are within the paper and its Supporting Information files.įunding: This work was supported in part by the NIH grants (RR020839 to JQ GM076102, CA160036, HG006434, GM111514, and CEIRS to HZ DK073368 and CA174423 to JZ). Received: MaAccepted: AugPublished: September 22, 2015Ĭopyright: © 2015 Hu et al. PLoS Comput Biol 11(9):Įditor: Andrey Rzhetsky, University of Chicago, UNITED STATES Furthermore, we propose two possible regulatory mechanisms by which the activity of scaffold proteins is coordinated with their associated pathways through phosphorylation process.Ĭitation: Hu J, Neiswinger J, Zhang J, Zhu H, Qian J (2015) Systematic Prediction of Scaffold Proteins Reveals New Design Principles in Scaffold-Mediated Signal Transduction. ![]() Interestingly, a single scaffold protein can be involved in multiple signaling pathways by interacting with other scaffold protein partners. We found that the scaffold proteins are likely to interact with each other, which is consistent with previous finding that scaffold proteins tend to form homodimers and heterodimers. The predicted scaffold proteins showed several interesting characteristics, as we expected from the functionality of scaffold proteins. The computational prediction was validated using a protein microarray-based approach. We predicted 212 scaffold proteins that are involved in 605 distinct signaling pathways. In this study, we performed a systematic analysis of scaffold proteins in signal transduction by integrating protein-protein interaction and kinase-substrate relationship networks. Scaffold proteins play a crucial role in facilitating signal transduction in eukaryotes by bringing together multiple signaling components. ![]() Foodi ninja chicken wings12/12/2022 ![]() ![]() FOODI NINJA CHICKEN WINGS CODECoupon code can only be used once per order. To save 15% on orders of $100 or more, enter PREP15 in the coupon code box during checkout on. Orders cannot be shipped to PO boxes or Hawaii, Alaska, Puerto Rico or other territories outside of the contiguous 48 states. SharkNinja reserves the right to cancel or modify this offer at any time. This offer is not valid with any prior purchases.
Compare fit bits12/12/2022 ![]() ![]() Now there multiple varieties of Fitbit available to suit many different groups of customers. It’s hard to believe that the first product Fitbit (opens in new tab) released back in 2009 was a clip-on device that only measured steps and sleep. The Apple Watch 7 has a slightly larger screen, but otherwise wasn't a huge upgrade from its predecessor (Image credit: Apple) There's a 70% brighter always-on screen mode for use indoors, the display is topped with tougher crystal, and the watch's case is now rated IPX6 for protection against dust. ![]() It retains the 7's slightly larger case sizes (41mm and 45mm compared to 40mm and 44mm for the Apple Watch 6). Compare fit bits skin#The Apple Watch 8 is a relatively iterative upgrade, with the main difference being the addiction of new functions like Crash Detection to detect car and bike accidents, and skin temperature sensors. Fitness is the primary function of the Apple Watch in 2022, so if that's your main concern then you won’t go far wrong here. The Apple Watch 6 and above include many of the same features, including blood oxygen monitoring, plus an on-demand ECG. The SE, especially the latest version, is a great entry point for those who want an Apple Watch with a heart rate feature at the lowest price. ![]() All are fundamentally excellent smartwatches that benefit from a mature operating system, so it's a matter of balancing your budget against their features. It’s best to see the Apple Watch versions as good, better, and best. All of the above use Apple's latest operating system for wearables, watchOS 9, which introduced new mindfulness tools, improved outdoor cycling features (including more accurate calorie counting for e-bikes), a pilates workout mode, and an updated photos app with new portrait watch faces. ![]() Decibel meter online12/12/2022 ![]()
Budweiser bigger picture12/11/2022 ![]() Many of these advertisers experienced significant growth during the pandemic. When you tune into the Big Game this Sunday, you're likely to notice familiar brands making their Super Bowl debut. Given the events of the past year, it's understandable why brands are erring on the side of caution with their messaging or choosing to forego advertising in the Super Bowl altogether. The brand took a social media-centric approach with over 90% of its $600,000 spend split between Twitter, Facebook, Instagram, and Youtube. The brand’s “A Nut Above” campaign aims to recognize people who step up to help in their community, whether it be a couple who donated the catering of their canceled wedding to the less fortunate or someone who paid a neighbor’s overdue rent.Ī Nut Above campaign was at the center of the 73 creatives ran through January 25th and February 4th. Planters is giving away the $5 million it would have spent on a Super Bowl ad spot and using it to recognize people doing charitable acts. ![]() Likewise, their campaign to promote the Kia Sorrento shows over half of their ad spend was funneled into news and trade websites. In the week leading up to the Big Game, Kia’s top creatives don’t hint at anything Super Bowl related. But after one hell of a rough year (pandemic), the brand realized America needed something much more than another ad in the Big Game. Kia’s decision to sit on the sidelines comes shortly after a significant investment in New Year’s Eve marketing. Budweiser Bigger Picture Sarah Dembkowski Budweiser is known for its Super Bowl ads. In addition to Kia, Audi, BMW, Lexus, Porsche, Mercedes-Benz, Volkswagen and Ford are among the auto brands who have decided not to run any Super Bowl ads this year. Kia also announced it would break its 11-year Super Bowl streak, citing a shift in priorities during COVID-19. Kia joins other auto brands in sitting out this year’s big game But this year, the biggest ad for the big game was one that didn’t air at all. Unlike Budweiser, Coors opted for an aggressive push across social, throwing 54% of their ad spend behind Instagram and 90% total across social channels. The subjects of the study were awoken and asked what they were dreaming about (spoiler alert: it involved cracking open a cold one). They released a spot that takes the viewer into a sleep study where participants are shown images and videos related to Coors. What’s your favorite spot from Super Bowl LV Be the critic on Fan Reviews Search. So, for the first time in 37 years, Budweiser sat out their in-game Super Bowl spot and instead relocated the media dollars to COVID-19 education and awareness. ![]() The video ends by plugging the Ad Council’s public education campaign on the coronavirus vaccine and promising viewers the beer juggernaut would "see at the game next year." From January 25th to February 4th, Bigger Picture dominated Budweiser's ad spend with 70% funneled into Youtube ads with Facebook trailing behind.Ĭonversely, their competitor Morson Coors took a different approach to Super Bowl advertising. Check out the Budweiser 'Bigger Picture' Super Bowl 2021 commercial. Budweiser is known for its Super Bowl ads. ![]() The ad, titled "Bigger Picture," highlights Americans coping under the strain of the pandemic. Instead, Budweiser's parent company, Anheuser-Busch, is donating its Super Bowl piggybank to coronavirus vaccination awareness efforts.īudweiser is promoting this historic move with a digital ad narrated by Rashida Jones. So what are they doing instead and does this signal a shift in brands moving away from Super Bowl advertising for good? Anheuser-Busch focuses on vaccination campaignīudweiser announced it would drop ad time in the Super Bowl for the first time in 37 years. ![]() Instead of shelling out a cool $5.5 million for a 30-second spot in Super Bowl LV, big-name brands are opting to spend that money elsewhere.Ĭommercial veterans like Budweiser, Kia, and Planters among others announced they would sit out this year’s Super Bowl. In early 2021, Budweiser, a beer brand, launched a campaign in the US with the goal to normalise the act of getting vaccinated to help get. This year’s Super Bowl will be remembered by a noticeable absence of household brand names. It consists of 1 video and 1 image.Super Bowl Sunday is the one day Americans not only tolerate ads-they’re a can’t miss part of the game-day experience. The piece was submitted to the medium: Public Relations within the entry type: Product/Service and the category: Cause Related. The advertising agency is part of the agency network: Ogilvy & Mather and holding company: WPP Group. This 2020 Clio Awards Shortlisted entry titled 'Budweiser Bigger Picture' was entered for Budweiser by David Miami, Miami. Yvette Cobarrubias / Cosmo Street EditorialĮxecutive Vice President, Consumer Brands ![]() Mazatlan zona dorada12/11/2022 ![]() ![]() ![]() This isn’t just a city that thrives during the day either, as there is plenty to do of an evening as well. it has Gothic arches, a golden Baroque altar, and rounded Renaissance domes. The Catedral Basílica de la Inmaculada Concepción is one of the most historic sites of Mazatlan. If you love history, there are few museums in the Centro to explore such as Museo Que Viva Sinaloa, Pedro Infante Museum, Museo Arqueológico, and Museo de Arte / Art Museum, and Archaeological Museum of Mazatlán. Surrounding them are beautiful parks where you can take a stroll to relax and fabulous markets where you can buy local food and wares. Those that don’t contain hotels now feature coffee shops, craft and souvenir stores, museums, and restaurants, giving you options on what to do with your day. Many of these buildings now house hotels and guest houses, where rooms are available for an incredibly affordable price, meaning you can completely immerse yourself in your surroundings and live a piece of history, no matter your budget. The city’s cathedral and the beautiful square that accompanies it are perhaps the main features of the district, but with the stunning Angela Peralta theatre, the glorious Plazuela Machado square, and numerous other historic buildings, including colonial mansions near narrow cobblestone streets that dating back as far as 500 years. The historic center also has wide range of tourist amenities such as hotels, restaurants, bars, and shops. Gamma Mazatlan The Inn At Centro HistoricoĬentro Historico is the best area to stay in Mazatlan for tourist, especially for first-timers because it is the true heart of the city, featuring its richest history and the majority of its most famous attractions. However, this time its beachfront location provides more of the things you would expect from a quiet seafront town. The three neighborhoods of Los Pinos, Loma Linda, and Olas Atlas are located to the west of Centro Historico and again feature a fairly quiet, local feel. Playa Norte is located to the north and features many of the same things as Playa Sur, just with a few more attractions, such as a baseball stadium and an aquarium. Playa Sur is located to the south of Centro Historico and is a largely residential district, where you can enjoy a local vibe and stunning views of the ocean from its hilly streets. While Zona Dorada is a very loud, full-on destination, Sabalo Country offers most of the same conveniences in a slightly quieter location. If you are looking for where to stay in Mazatlan all inclusive, look into Zona Dorada because it’s home to many high-end hotels, busy beaches, and bustling shops, bars, and restaurants. Meanwhile, Zona Dorada along with Sabalo Country is a part of Golden Zone. Centro Historico is filled with many historical and cultural attractions, as well as hotels, restaurants, and bars, it is the best place to get a feel for how the city has changed over the years. Known as Pearl of the Pacific, Mazatlán is also a beach resort tourist town with beautiful beaches and amazing weather especially in winter, it attracts lots of United States and Canada from November to April.Ĭentro Historico along with Zona Dorada is the best area to stay in Mazatlán for first-timers. Mazatlán is the colonial beach city, located in the state of Sinaloa, on the western coast of Mexico on the Pacific Ocean. In today’s article, I will be looking at the safest neighborhoods in Mazatlán, where to stay in Mazatlán and best hotels in each area. The best areas to stay in Mazatlán for first-timers are the neighborhoods of Centro Historico, Playa Sur, Los Pinos, Playa Norte, Golden Zone, Sabalo Country, Marina Mazatlán, Nuevo Mazatlán, and Cerritos. ![]() After opal12/11/2022 ![]() ![]() Common opal does contain tiny balls of silica dioxide, but they are irregular in shape, size and/or arrangement. Common opal is typically grey, black, white or amber-coloured, but is also found in other hues. The great majority of opal does not show play of colour and is called common opal or potch. The tiniest spheres produce violet-coloured opal the largest, red, with sphere size increasing from the violet to the red end of the spectrum. This configuration creates lattices that diffract white light into different colours of the spectrum. In precious opal – the name given to opal with spectral colours, or 'play of colour' – the tiny spheres of silica dioxide are uniform in size and stacked in regular rows and layers. Opal is made of tiny spheres of silica dioxide - spheres so tiny they can only be seen using an electron microscope. Each location produces a distinctive opal type in the future, we will add photos and information about these to the Australian Opal Centre website. Opal is found in several other countries including Brazil, Mexico, Ethiopia, the United States, Canada, Peru, Indonesia, Honduras, Slovakia and the Czech Republic. ![]() In 1994, opal was declared Australia’s National Gemstone. Australia is regarded as the world's leading source of opal and of the world's finest opal. Much of the world’s precious opal is mined in the harsh outback of Australia, where a unique combination of geological conditions permitted the formation of opal near the margins of an ancient inland sea. Like all fine things, gem opal is exceedingly rare. It boasts every colour of the visible spectrum, from deepest and clearest blues and greens to rippling golden orange through delicate pink and violet to rich turquoise, shocking vermilion, carmine and fuchsia - every colour imaginable.Īn opal may contain any or all of these colours, arrayed in wondrous patterns with names like harlequin, pinfire, Chinese writing, flower garden, mackerel sky, flagstone and rolling flash. Opal is the most magnificent of precious gemstones. ![]() AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |